ocelloscope detection system  (Biocell Technology)

Journal: LWT

Article Title: Inhibitory effects of binary combinations of microbial metabolites on the growth of tolerant Penicillium roqueforti and Mucor circinelloides

doi: 10.1016/j.lwt.2021.112039

Figure Lengend Snippet: Fig. 3. Growth inhibition curves and oCelloScope images of morphological changes of spores and mycelia of P. roqueforti ISI4 (A, B) and M. circinelloides 01180023 (C, D) induced by the compounds in MEB medium, respectively. Measurements corresponding to images are marked on graphs by a dotted rectangle. The con centration of each compound used in this assay was 1/2 × MIC. Error bar represents the mean value of three replicates. Scale bar: 50 μm. 3-PPA: 3-Phenyl propanoic acid.

Article Snippet: Growth kinetics analysis and morphological changes Mold growth inhibition and morphological changes were monitored at 25 ◦C using the oCelloScope Detection System (BioSense Solutions ApS, Farum, Denmark) as previously described (Aunsbjerg, Andersen, & Knøchel, 2015).

Techniques: Inhibition

Journal: Scientific Reports

Article Title: Growth and single cell kinetics of the loricate choanoflagellate Diaphanoeca grandis

doi: 10.1038/s41598-019-50998-0

Figure Lengend Snippet: Diaphanoeca grandis . Number of individuals in filmed area in oCelloScope, grown on 3,000 (◇), 6,000 (○) and 12,000 (□) Pantoea sp. μL −1 . Inset. ln( D. grandis numbers) vs. time, exponential growth phase indicated by solid symbols, slope of regression line equals specific growth rates, µ (Supplementary Information, Table ).

Article Snippet: Growth of Diaphanoeca grandis was also monitored at the single-cell level in the oCelloScope optical detection system (Biosense Solutions Aps, Denmark), which is an automatic digital microscope equipped with a 4x optical magnification (200x effective magnification) , .

Techniques:

Journal: Scientific Reports

Article Title: Growth and single cell kinetics of the loricate choanoflagellate Diaphanoeca grandis

doi: 10.1038/s41598-019-50998-0

Figure Lengend Snippet: Diaphanoeca grandis . Data on cohorts of individuals inoculated and filmed in the oCelloScope and grown on 3,000 (38 individuals, A – D ), 6,000 (40 individuals, E – H ) and 12,000 (40 individuals, I – L ) Pantoea sp. μL −1 . A , E and I . Number of cell divisions within 12 h time intervals. Solid bars indicate first release of a daughter cell, open bars indicate release of subsequent daughter cells. B , F and J . Number of inoculated D. grandis that have not yet carried out a cell division. C , G and K . Time span between cell divisions, excluding the first cell division (○), τ 1 and time span between replacement of a lorica and the following cell division (●). D , H and L . Number of protoplasts leaving and replacing their lorica within 12 h time intervals.

Article Snippet: Growth of Diaphanoeca grandis was also monitored at the single-cell level in the oCelloScope optical detection system (Biosense Solutions Aps, Denmark), which is an automatic digital microscope equipped with a 4x optical magnification (200x effective magnification) , .

Techniques:

Journal: Scientific Reports

Article Title: Growth and single cell kinetics of the loricate choanoflagellate Diaphanoeca grandis

doi: 10.1038/s41598-019-50998-0

Figure Lengend Snippet: Diaphanoeca grandis . Characteristic life times (average values ± standard deviation) of individual cell stages from 3 cultures grown in the oCelloScope on Pantoea sp. (Figs 5 and 6 ). Time span between cell divisions, τ 1 . Life time of stage with two cells (mother and daughter) inside the parental lorica, τ 2 . Life time of motile, non-loricate cell stage, τ 3 . Time expenditure for assembly of new lorica, τ 4 . Time span since last cell division when a protoplast abandons its lorica, τ 5 . Number of events, n .

Article Snippet: Growth of Diaphanoeca grandis was also monitored at the single-cell level in the oCelloScope optical detection system (Biosense Solutions Aps, Denmark), which is an automatic digital microscope equipped with a 4x optical magnification (200x effective magnification) , .

Techniques: Standard Deviation

Journal: Scientific Reports

Article Title: Growth and single cell kinetics of the loricate choanoflagellate Diaphanoeca grandis

doi: 10.1038/s41598-019-50998-0

Figure Lengend Snippet: Diaphanoeca grandis . Data on cohorts of individual D. grandis inoculated and filmed in the oCelloScope and grown on 3,000 (38 individuals, A – C ), 6,000 (40 individuals, D – F ) and 12,000 (40 individuals, G – I ) Pantoea sp. μL −1 . A , D and G . Life time of stage with two cells (mother and daughter) inside the parental lorica, τ 2 . B , E and H . Life time of motile, non-loricate cell stage, τ 3 . C , F and I . Time expenditure for assembly of new lorica, τ 4 . Open symbols indicate events following cell divisions. Closed symbols indicate events that occurred after a protoplast had abandoned its lorica.

Article Snippet: Growth of Diaphanoeca grandis was also monitored at the single-cell level in the oCelloScope optical detection system (Biosense Solutions Aps, Denmark), which is an automatic digital microscope equipped with a 4x optical magnification (200x effective magnification) , .

Techniques: